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Berkala Penelitian Hayati
Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 11 Documents
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Search results for , issue "Vol 16 No 1 (2010): December 2010" : 11 Documents clear
STRUKTUR ANATOMI DAN AKTIVITAS ANTIOKSIDAN BULBUS BAWANG DAYAK (Eleutherine americana MERR.) DARI DAERAH KALIMANTAN SELATAN Evi Mintowati Kuntorini; Maria Dewi Astuti; L. Hartanto Nugroho
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 1 (2010): December 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/276

Abstract

The aim of this research were to study the characterization of the microscopic anatomy and testing the antioxidant activity of bawang dayak bulb from several regions in South Kalimantan. Bawang dayak plant samples taken from four (4) regency in South Kalimantan. Bulb anatomical structure was observed by the paraffin method and test preparations of antioxidant activity by DPPH method. IC50 values were calculated based on the formula of the regression equation.The bulb anatomical structures has an epidermis tissue of both surfaces, there is parenchymal tissue. Transport tissue were located in rows with collateral type, there are starch grains in parenchyma cells, and the presence of stiloid crystals between cells parenkim. Extract ethanol bawang dayak bulb from the four districts in South Kalimantan has antioxidant activity against DPPH radicals. The highest antioxidant activity showed on the sample from location1 Comets Village Banjarbaru Municipality (IC50 = 25.3339 μg/ml) and the lowest showed on the sample from location 2 Sungai Paring Village Banjar District (IC50 = 86.9039 μg/ml). Antioxidant activity of bawang dayak extract 4.5 to 15 times weaker compared to BHT (BHT IC50 = 5.5707 μg/ml).
KARAKTERISASI EKSTRAK KASAR FITASE TERMOFILIK DARI BAKTERI KAWAH IJEN BANYUWANGI, ISOLAT AP-17 Aline Puspita Kusumadjaja; Tutuk Budiati; Sajidan; Ni Nyoman Tri Puspaningsih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 1 (2010): December 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/277

Abstract

Crude thermophilic phytase was produced by isolate AP-17 that has been isolated from Ijen Crater Banyuwangi. Based on Gram test, isolate AP-17 was gram positive spore forming rod shape bacteria so that it was identified as Bacillus sp. AP-17. Crude thermophilic phytase isolated from Bacillus sp. AP-17 had the optimum temperature at 75° C with activity of 0.1413 U/ml, and its optimum pH was at pH 6 with activity of 0.0875 U/ml. The enzyme was stable when heated at 75° C for three hours and still had 90% activity when it was exposed at pH 5–8, optimum temperature, for one hour.
POLA DOMINANSI CAPIT PADA Uca spp. (DECAPODA: OCYPODIDAE) Dewi Citra Murniati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 1 (2010): December 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/278

Abstract

Uca spp was a unique crab species marked with asymmetry of the cheliped. A major cheliped and a minor cheliped has different function. Major cheliped size is several times of minor cheliped size (cheliped dominance pattern). Each species has different cheliped dominance pattern. This study was done to describe cheliped dominance pattern of three Uca species. Major cheliped length and minor cheliped length of twenty male of U. dussumieri, U. vocans and U. perplexa were examined. Cheliped dominance pattern obtained by compared the major cheliped length to minor cheliped length. The two-tailed single sample t test showed similar cheliped dominance pattern within Uca species (P > 0.001). The Kruskal-Wallis test showed different cheliped dominance pattern among Uca species (P>0.001). The cheliped dominance pattern of U. dussumieri, U. vocans and U. perplexa are 5.09, 4.24 dan 4.75. Size and morphology of manus, cheliped finger and cheliped gap result in total length of cheliped.
PERANAN Ulva pertusa DALAM MENURUNKAN PENYERAPAN TIMBAL (PB) OLEH KERANG DARAH (Anadara granosa) DAN MENEKAN PERTUMBUHAN DINOFLAGELLATA (Noctiluca miliaris) Moch. Amin Alamsjah
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 1 (2010): December 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/279

Abstract

Profile of shell with toxic and non-toxic are not different, whereas these toxin is caused by absorption of lead from contamination water and bioactive compound of Dinoflagellata.The other side, an active compound of Ulva pertusa polyunsaturated fatty acid which has ability to potent for survivability of Dinoflagellata (Noctiluca miliaris). U. pertusa is also known to have capability as a biosorption in the sea. The results showed that the effect of algicidal substances U. pertusa was not influence on survivability of shell however it can decrease the growth of Dinofl agellata (N. miliaris) and to absorb lead. Quantifi cation analysis of fatty acid composition also showed that U. pertusa is dominated by PUFA as much as 59% of fatty acid total and has algicidal substances of HDTA, ALA, and ODTA were 1094.44 mg/100g. Analysis of lead in shell, U. pertusa and water showed that they have a significant correlation, where U. pertusa (400 g) can absorb lead until 78.17%, which the initial concentration of lead in U. pertusa and shell were 1.09 mg/kg and less than 0.14 mg/kg, respectively.
PENGARUH ASAM ASKORBAT TERHADAP KADAR TIMBAL FETUS DAN AKTIVITAS ENZIM SITOKROM P450 1A1 (CYP1A1) PADA INDUK MENCIT TERINTOKSIKASI TIMBAL Juliana Christyaningsih; Harianto Notopuro; Win Darmanto; Diah Titik Mutiarawati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 1 (2010): December 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/280

Abstract

Lead contamination occurs through air pollution and industry, enter the body through respiratory and digestive tract. High lead content will accumulate and affect adversely the cognitive function, causing neuropsychological dysfunction, encephalopathy, hyperactivity and other problems in children, disrupt the central nervous system and the immune system of children as well. This experimental research was randomized control group post-test only design. The experiment used of 27 pregnant mice, divided into three groups: negative control group, which were given distilled water, positive control group were exposed only to lead and the third group were exposed to lead and administered ascorbic acid. 25 mg/kg/day/orally neutral lead acetate was given during gestation day 7 to 16, and ascorbic acid 64 mg/kg/day/orally, started on gestation day 9 to 16. Treatment group with ascorbic acid supplementation had the lowest CYP1A1 enzyme activity compared to positive and negative control groups. This results confirmed by the molecular weight of CYP1A1 enzyme ranges 53.7 to 59.2 kDa, and the western blotting test showed the same thin band both two groups. The lowest of the average lead concentration in the head of fetal mice was found on the group of mice that treated with vitamin C. Supplementation of ascorbic acid can protect the liver and fetuses, by suspected mechanism that ascorbic acid could chelate the lead and excrete it via urine.
STUDI KINETIKA PRODUKSI BIOSURFAKTAN Bacillus subtilis 3KP PADA SUBSTRAT MOLASE Ni’matuzahroh; Nur Hidayatul Alami; A. Faiz Khudlari T.; Fatimah; Tri Nurhariyati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 1 (2010): December 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/281

Abstract

Bacillus subtilis 3KP was a bacteria that can produce biosurfactant, when it was grown on a molasses substrate. This research was aimed to know the kinetic type of biosurfactant production, so it can be known the efficiency of molasses and the optimal culture condition to produce biosurfactant. B. subtilis 3KP was grown on mineral salt medium that had been already added with various molasses concentrations (1, 2, 3, and 4 g/l). The culture was incubated until 7 days on a shaker at 27° C and 120 rpm. The measurement of bacterial growth (CFU/ml), pH culture changing, and biosurfactant production which was indicated from emulsification activity and crude biosurfactant weight was determined at various incubation periods (0, 1, 2, 3, 4, 5, 6 and 7 days). Emulsifier activity (E1h) was measured by adding 1 ml of kerosene to 1 ml of biosurfactant supernatant. The measurement was made after 1 hour. The E1h index was given as percentage of height of emulsified layer (cm) divided by total height of the liquid column (cm). Crude biosurfactant was got from ammonium sulfate precipitation, then was dried and was determined in (g/l culture). The data of growth and biosurfactant production were analysed descriptively to know differentiation of biosurfactant production type of B. subtilis 3 KP in that different treatment. The examination of kinetics parameters such as product yield (Yp/x) and specific rate of culture (μ) were taken into account to evaluate a high efficient production process. The results of this research indicated that biosurfactant of B. subtilis 3KP was begun produced at exponential phase and was optimal at the end of exponential phase (production under growth-limiting conditions). Molasses concentration of 3% and 4 days incubation gave an optimal biosurfactant production, so it can be became the exact combination alternative to scale up biosurfactant of B. subtilis 3KP.
INVENTORY OF Musa paradisiaca L. (BANANA) KEPOK IN LUMAJANG REGENCY, MALANG REGENCY AND MAGELANG REGENCY Suhadi
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 1 (2010): December 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/282

Abstract

Banana is fruit containing fairly high nutrition and provides quick reserve energy. The crop grows in tropical area with average rainfall all the year and banana produces at any season. One of the bananas which has high value sale and high competable potency is subvariety of kepok banana. Kepok banana has various subvarieties, these subvarieties have the same morphologies but have different texture appearances thus uneasy to differenciate among them. The texture appearance determines the quality and price of the banana. Often the buyer makes a mistake in choosing subvariety of kepok he wants to, whereas the seller gives him the cheapest subvariety of kepok. Methods we used was method of exploration using free exploration technique step by step without any certain path. There were two phases in the research namely the first phase was carried out in field and the second phase was done in the laboratory. Subvarieties of kepok found in Lumajang Regency are 4 subcultivars, Malang Regency there are 3 and Magelang Regency are subcultivars subcultivars, The sequence of the quality of kapok subcultivars are as follows, red kepok, yellow kepok, big (gede, gilo, gembrot) kepok, and white kepok. Sugestion, organic ferlitilizer should be used in the fertilization of banana cultivation, and conservation of red kepok is highly required.
SOME COMMON SPECIES OF PLANKTONIC HARPACTICOIDA (CRUSTACEA, COPEPODA) FROM INDONESIAN WATERS Mulyadi Ningsih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 1 (2010): December 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/283

Abstract

Taxonomic study was made on the species of the order Harpacticoida recently collected from 9 sites in Indonesian waters. Six species from 5 genera and 5 families Clytemnestridae, Ectinosomatidae, Harpacticidae, Tachidiidae and Talestridae, including Clytemnestra scutellata (Dana, 1847); Euterpina acutifrons (Dana, 1848), Eudactylopus latipes (T. Scott, 1894); Macrosetella gracilis (Dana, 1848); Microsetella norvegica (Boeck, 1864) and Microsetella rosea (Dana, 1852) were recorded. C. scutellata and M. norvegica are recorded for the first time from Indonesian waters. Descriptions, measurements, and figures are given for all species, along with a review of their distribution over the world’s oceans, with taxonomical remarks, and restricted synonymies
THE DIVERSITY AND RICHNESS OF TREE SPECIES OF TAMBANG SAWAH FOREST, KERINCI-SEBLAT NATIONAL PARK, SUMATRA INDONESIA Agus Susatya
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 1 (2010): December 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/284

Abstract

The conservation of tropical ecosystem is increasingly relevant as the recent global warming and climate change generate serious impacts on human life. Tropical forest becomes an important ecosystem to fight global warming due to its capability to sequester atmospheric carbon and to mitigate climate change. It is very unfortunate that such a vital ecosystem has been severely subjected to conversion to both plantations and illegal loggings. The tropical ecosystem has long been recognized to have high species diversity, but very few individual trees per species. The latter is almost ignored, even though can certainly bring serious difficulties on tree conservation. The objectives of the research were to know the tree community structure of Tambang Sawah Forest, Kerinci-Seblat National Park, and to determine the rareness of tree species. A plot of 1 ha was established at Tambang Sawah, Kerinci-Seblat National Park, Lebong Regency. All trees with BDH of > 5 cm were collected their herbarium specimens, and identified. The results showed that Tambang Sawah forest consists of 42 families, 94 genera, and 185 tree species/ha. It has 19.51% (8 families), and 26.82% (10 families) respectively categorized as very rare and rare. The pattern also occurs at genus level, where both categories contribute to 81.91% (78 genera) of the total genera. In species level, both are respectively 90 and 28 species, and altogether contribute to 63.78% of the total species. These values appeared higher than that of the other forests in Bengkulu. Across taxon level, very rare and rare categories appeared to be an ecological attribute in Sumatran forests. This implies that the loss of single tree can cause the loss of entire family. The conservation works even turn into more difficult, because tropical trees are commonly diocious, even bisexual trees, they tend to be self-incompatible, and out-crossed, and required at least 200 mature trees to ensure sexual regeneration and to avoid species extinction.
MORFOLOGI FUNGSIONAL KERANG BATIK Paphia undulata (Bivalvia: Veneridae) Reni Ambarwati; Trijoko
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 1 (2010): December 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/285

Abstract

Veneridae bivalves are well-known and have economic significance, consumed as food and used for ornaments. Paphia undulata is one of the members of Veneridae. However, the functional morphology of this species has not been studied. The purpose of this research was to study the morphological characters of Phapia undulata. Specimens collected from Sidoarjo Coastal Water, Jawa Timur. Specimens relaxated by using MgCl2 7% in sea water and fixed in 10% formalin in sea water. Finally, specimens were preserved in 70% ethanol. Observation was done on the morphology internal of all specimens. Morphometric measurements were done on the length, height, and width of shells, length and width of ctenidium and labial palp. The results of this research showed that the diagnostic characters of P. undulata ornament of the shell, form and the type of siphon, and the form of foot. Ratio length: height of the shell = 1:0.6; ratio length = width of the shell: 1:0.4, ratio height: width of the shell = 1:0.6. Based on the morphometric measurements of ctenidium and labial palp, P. undulata can be categorized as suspension feeder. The ratio of ctenidium: labial palp = 5:1.

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